Old Immunology Exam(s)

MMI4804.- 2004 Quiz 5 1.

SelecttheINCORRECTstatement aboutHIV cytopathiceffects. at thecell surface Eg to CD4 insidethecell blocksCD4 expression

C. D. E.

2.

select the INCORRECT statementconcerning Type I Immediate (Anaphylactic) Hypersensitivify. A.

D. E.

3.

vpu aid nef slnc productsuprcgulateCD4 and MHC ClassI Syncytia formation kills both infectedand uninfectedcells Increase in plasma membranepermeability results in the uptake of lethal amounts of calcium (osmotic lysis) vRNA and unintegratedvDNA accumulationis toxic to cells

Antigen binds to IgE on the surfaceof mast cells causingthe releaseof inflammatory mediators. ires the formation of IgE antibody. ipg specific IgE that is firmly artachedto lrceRl on mast or activatedeosinophils Re-exposureto the sameantigenresultsin cross-linking of the cell-boundIgE and release of inflammatory mediators within minutes. Crosslinking of FceRI on the surfaceof mast cells by antigenand IgE causes mast-cell activation and degranulation.

Selectthe INCORRECT statementconcerninsAutoimmune Diseases.

A. B. C. D.

Autoimmune diseasesare chronic inflammatory responsesdirectedagainst components of the body. Immunity againstself antigens(called autoantigens)is called autoimmunity. They are much more prevalent in females. 2-3% of the population of developedcountriessuffers from an autoimmune cttrbIe.fypcihypcrsensitivityrcactions.

+.

moleculescausingthe immune responseto be Some tumo$ secreteimmunosuppressive TRUE down-regulated.

5.

In Myasthenia Gravis, antibodies bind to the acetylcholine receptor on muscle cells resulting in their endocytosisand degradation. TRUE

6.

Rabies fmmune Globulin EiC) is used for prevention of rabies for those exposedto the virus and is producedby bleeding feral rabid dogs, squirrelsand horsesthat have bitten humansFALSE

7' The phenomenonin which further immunogrobulinrearrangement is shutdown on theotherchromosomal alleleis called A. Clonal selection B. Allelic imprinting 1e)ettetic exitusion Y. Isotype switching E. Productive rearrangement 8' The processby which neutuophilscross the vascularendotheliumis called LA-2diapedesis ," .; B. chemotaxis C. complement fixation D. opsonization E. polymorphonuclearleukocyteresponse 9'

which of the following is not a characteristic of macrophages? A. secretionof soluble mediators I killing tumor cells €) rearrangement of antigen-specifi c receptors D. phagocytosisof immune complexes E. presentationof antigento T cells

I0' which combination of cytokinesact in the chemotaxisof polymorphonuclear leukocytes (pMNs)? A. IL-2 and IL-4 @ IL-$ and llrlFcr C. L-10 and IL-12 D. IL-4 and IL-5 E. none ofthe above

1l. Membrane-boundIgM characterizeswhich of the following cell types? A. B. (Q Y E.

T helper cell plasrnacell i^-unocompetent B cell cvtotoxicT cell dendritic cell

12. Which of the following would be a CD4 positive cell? A. plasma cell B. cvtotoxic T cell $ thelper cetl D. langerhans cell E. macrophage 13. Which is the order of a pathogenthat crossesthe epithelialbarrier in high concenhation? connective tissue > peripheral lymphatics > lymph node > vasculature > spleen connectivetissue> peripherallymphatics> spleen vasculature > spleen> peripheral lymphatics > lymph node connective tissue > peripheral lymphatics > lymph node > thymus > vasculature> spleen E. vasculature > spleen > lymph node

@ B. C. D.

*d 14. Which of the following best describesthe differencesbetweenhow T cells and B cells recognize antigen? A. T cells recognizeself MHC molecules,while B cells recognizesoluble antigen. Q f ce[s rccognize foreign peptidesin the context of self MHC molecules,while B cells recognize native, foreign proteins. C. T cells rccognizeprotein antigens,while B cells recognizenon-proteinantigens. D. T cells recognizeforeign peptides,while B cells recognizewhole proteins. E. T cells recognize self MHC molecules,while B cells recognizepeptide antigens.

15. Peptideloadingof MHC classII moleculeswith foreignantigenoccurs - {\r. endosomal/lysosomal compartment @)in B. in cytosol C. on cell surface D. in endoplasmic reticulum E. in golgi 16. T cell development in thethymusincludesthefollowingprocesses: : A. T cell receptorrearrangement B. negativeselection q positive selection *@ all of the above E. only B and C '.

- ' 17. After degradationin the cytoplasm,fragments of foreignproteins(ie virus-derived) arepumpedinto theendoplasmic reticulumfor MHC classI peptideloadingwith helpof..-. A. invariantchain Q proteasomecomplex I €)Transporter Associated with AntigenProcessing (TAp) D. MHC classII E. None of the above l8.Susceptibilitytoautoimmunediseaseisinfluencedby-. A. B. (!) D.

geneticfactors environmentalfactors botUA and B noneof the above

' '"' 19' Which of the followingautoimmune diseases is characterize
rheumatoidarthritis systemiclupuserythematosus (SLE) Hashimoto's disease multiplesclerosis noneofthe above

20' T cell autoimmunityagainstmyelinantigenscanleadto which autoimmune disease? multiple sclerosis myastheniagravis insulin-dependentdiabetesmellitus (IDDM)

D. rheumatoidarthritis E. none ofthe above

2l ' Microbial infections can precipitateautoimmunediseases in what way(s)? \!J molecular mimicry

B. pathogen-induced tissuedarhage. releaseof autoantigens ^A of theabove <--€lall E. none ofthe above t' 22' Mechanismsof peripheral toleranceinclude all of the following EXCEpT

A. B. L. \D) E.

Activation inducedcell death CTIA-4 signaling Productionof suppressive cytokinesby regulatorycells Destructionof autoreactive cellsbv CTLs Clonal anergy

2

23. Which of thefollowingis NOT a functionof T helperI (Thl) cells? A. B. C. @ E.

Productionof IFNy Activation of naturalkiller (NK) cells vialL-2 production" Promotionof cytotoxicT cell differentiation pro-otion of IgE productionby B cells , r..: Activationof macrophages anddendriticcells.

24. Which of thefollowingcostimulatory moleculesbind to 87 on antigenpresenting cells? A. CD28 B. CTLA4

c. cD40

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D. all of the above @>both A and B ,,. 25. Which of the following statementsis TRUE regardingnarveT cells? A. Naive T cells circulatethroughoutthe entire body, including the spleen,and readily leave the intravascular space. B. NarVe T cells produce large amounts of effector cytokines such as IFNy and IL- t ,

t2. Naive T cells expressT cell receptorbut have not been exposedto antigen. D. Naive T cells are extremelyshort lived, with an averagesurvival time of 8-12

hours. for activation.. E. Naive T cellsrequirelittle costimulation areTRUE regardingmemoryT cells? 26. Which of the following statements A. Memory T cellshavean extremelylong life span. B. Memory T cellsarederivedfrom the populationof effectorT cells' C. IvtemoryT cells requirelittle costimulationfor proliferationandcytokine production. D. Memory T cellscirculatethroughthe entirebody andreadilyleavethe - intravascularspace. @eu of the above

27. Which of thefollowingdescribetherole of CD28in T cell activarion? A. CD28on theT cell bindsto CD4OI,on theantigenpresenting cell. B. Activationof CD28promotescell death. C. Preferentialengagement of CTLA-4 versusCD28may leadto cell proliferation. D. Only A and B are correct p. A, B, and C are correct j fF\, None of the above '.u , L-,/ . 28. The bone marrow is an imoortant site for ''..^\ r '-j-.!&) hematopoiesis 1 B. B cell maturation C. T cell maturation D. immune surveillance E. central tolerance

-

,, 29. Which of the following doesnot protectthe externalbody surfaces? A. B. :-l-9. -Xal E.

mucous.' skin gutttic acid salivary.amylase gut microflora

30. The processby rvhichcomplement enhances phagocytosis of bacteriais called ,^\ ' 0.) opsoruzatron. K-chemotaxis C. exocytosis D. proteolysis E. bacteriolysis

3l ' which oneof theseconditionsis not directryassociated with HIV?

A. Profoundimmunosuppression B . OpportunisticinfectionsandCNS degeneration

I

Multiple sclerosis Malignancies andwasting

32. Which statementis incorrect about CCR5? A

Is expressedon dendritic cells, macrophages,CD4+ T cells 1 r' r* r, Appears late in diseaseand is the onlt'coreceptor for HIV @ 9. the receptor used by the most frequently transmitted virus (mucosal route) ls D. Servesas coreceptorfor macrophage-tropicHIV strains 33. Which statementis incorrect about HIV pathogenesis?

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vpu and nef geneproductsupregulateCD4 Syncytia formation kills both infected and uninfectedcells c. HIV specific crl- attackcausean increasein plasmamembranepermeability results in the uptake of lethal amountsof calcium (osmotic lysis of infected cell) D . vRNA and unintegratedvDNA accumulationis toxic to cells

34. Which of the following is not an advantageof killed vaccines? A. B. . c. 'B

Cannot revert to virulence More heat stable can be safely given to immunodeficientpatientsand pregnantwomen L induces a weaker mucosal IgA antibody response

35. Which statementis not true about Subunit Viral Vaccines? A. Contain primarily the immunity-inducing component of the virion B. Consist of protein or portions of proteins from virus particles AContain active nucleic acid consiiting of the entire genome H. Can be natural or recombinant proteins

36. Which statementis not accurateabout Bacille Calmette-Guerin(BCG) Vaccine? It is a live attenuatedstrainof Mycobacterium&ovlsftovine strain) .A ' r" " rd',.r . 'r (U)Is routinely given in Europe, US and other parts of the world rn.' T. It invalidatesthe TB skin test usedto verify exposureto the tuberclebacillus D. Is effective againsthuman tuberculosis(TB)., 37. which statement is not true concerning Type I Immediate (Anaphylactic) Hypersensitivity? A. First exposureto antigen stimulatesproduction of IgE that bind to the surfaceof mast cells (8lB'l Results in formation of IgE antibody that is not antigen specific C. Antigen binds to antigen specific IgE that is firmly attachedto FceRI on mast cells, basophilsand activatedeosinophils D. Re*exposureto the sameantigencross-linksthe cell-boundIgE causingreleaseof inflammatory mediators within minutes (immediate reaction) 38. Which statementis not accurateconcemingatopy or allergy? _,. - ,."i

r ,.'

Lessthan l0% of people in the US have experiencedan atopic disorder

D. Genetic predispositionincludesmutationsin the genesencodingthe alpha chain of IL-4 that enhancesthe effectivenessof IL-4, resulting in increasedamount of IgE synthesisby B cells 39. Which statementis incorrectconcemingType IV Delayed-TypeHypersensitivity

(DrH)?

A. It is a function of T lymphocytesand can be transferredby sensitizedThl cells (CDt T ceils) B.' Responseis delayed and begins hours or days (peaks at24-48 hours) after exposure to antigen.. Q There is a slow inflammation at the site of antigenexposue .' fD) fne tuberculin test is always positive in people with suppressedcell-mediated rmmunity (AIDS, measles,lymphoma) .f,

40. which statementis not accurateconcerningtumor suppressorgenes? A. They encodeproteins that preventthe unwantedproliferation of mutant cells B. p53 is a fypical tumor suppressorgene and is muiated in 50% of human cancer cases C, They are expressedin responseto DNA damage gene encodesa protein that causesthe damagedcell to proliferate pnsl 41. Which one of thesevirusesis not associatedwith induction of human cancer? A. Human Hepatitis B Virus and Hepatitis C Virus B. Human Papillomavirus Virus C. Epstein-Barr Virus Virus @lnnu"nza 42. which statementis not true of the immune responseto T and B lymphomas? A. The T cell receptorexpressedby a T cell lymphoma can be a tumor specific antigen. B. The membrane immunoglobulin expressedby a B cell lymphoma can be a tumor antigen. ^specific papillomavirus virus (F{PV) can be a rumor specific antigen (p|ryan -D. Both cytotoxic T lymphocytes(CTLs) and antibodiescan r"cog.rize tumor specific antigens 43. which of the following.testscan not be usedto measurean antigen level? A. (E E D.

immunodiffusion complement fxation Ertsa Western blot "

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44.A 3 yearold presentswith oral ulcers,poordentition,anda glutealabscess which culturesS. aureus.You suspect he hasa primaryneutrophildefect.Considerations for diagnosisincludeall of thefollowingexcept.----.-. A. Job'ssyndrome B. leukocyteimmunodeficiency (LAD-1) syndrome tC)iGeorse svndrome. \--l^'

D. Chronic granulomatousdisease(CGD)

45. Which of the following patientswith recurrentinfections is most likely to survive to adulthood? A. severecombined immunodeficiency syndrome (SCID) Wiscott-Aldrichsyndrome 9.. rR\ common variable immunodeficiency syndrome (CVID) tC.) D. ataxiatelansiectasia 46. T cell number olr.,n"tion can be measuredby all of the following except A. B. C. D. @

flow c5rtometry delayedtype hypersensitivity(DTH) response lymphoproliferation cytokine nroduction ainyarorhodamineoxidation

****'t:l c***+******x*{ < **+*******,F**************,k * * {<* * * t<* * * :r * * i <* * * * * t

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For each of the following questions,please answer A if the statementis true and B if the statementis hlse.

47. In the acquired immune response,B and r lymphocytes only generatereceptor specificitiesJhat bind to previously encounteredforeign antigens.

4 8 . Each imrrunoglobulin molecule has 2 antigen binding sites that are each made from 4 polypeptide chains.

49. Mature T lymphocytes oeanng a particular T cell receptor will undergo somatic hypermutation and affinity maturation upon encountering foreign antigen (presented on self-MHC on antigen oresenting cells) in the periphery.

50. Naturat kilter (NK) celts uan kill turqor cells in the absenceof MHC class I. 51 . IL-2 can induce both apoptosisand prourbrationof T cells.

52. Transplantrejection occurswhen a transplanteddonor organ is recognizedas foreign and is attacked by the recipjqnt's immune system. 53. Immunoglobulin V heavy chain generecombinationis similar to T cell receptorB chain recombinationbecauspthey both use diversity (D) gene segmentsto make VDJ gene segments. 54. The gene segmentCe encodesthg constantregion of the IgD isotype. 55. The strongestgenetic factor inl^*--cing susceptibilityto autoimmunediseaseis inheritance of particular HLA allqles. 56. Inappropriateregulation of T helper cell responseslikely plays a role in a majority of autoimmunediseases. 57. SelectiveIgA deficiency is the most common inheritedimmunodeficiencyin the US population. 58. Detection of antigen specific antibodiesof isofypeIgM is routinely performed in clinical laboratories. 59. Cells destinedto becomeB cells arisetrom hematopoieticstem cells in the bone marrow, enter the circulation, and are directedto the thymus by chemotacticfactors. 60. Recurrentbacterial infections are alwayqpredictiveof inheritedor acquired immunodefi ciencv svndromes.

I I

MMI480A 2002Final Exam l.A 2.B 3.D 4.A 5.C 6.8 7.C 8.A 9.C 10.B ll.c 12.c 1 3 .A 14 .B 15 .A 16 .D 17 .C 18 .c 19 .B 20.A 2 t.D 22-D 23 .D 24 - E

2s . c 2 6 .E 27 .F 28.A 2 9 .D 30.A

3 1 .C 32.8 3 3 .A 34.D 3 5 .C 3 6 .B 37.8 3 8 .A 3 9 .D 40. D 4 1 .D 42.C 43.8 44.C

4s.c 46.8 47.8 4 8 .B 49.8 50.A 5 1 .A 52.A 5 3 .A 54.B 5 5 .A 5 6 .A 51.A 5 8 .B 5 9 .B 60.B

ffir\ MMI4SOA Spring 2002 Final Examination For eachof the following question,selectthe single best answerto fili in on your answer sheet.

l. Which of the following are true statementsconceming the differencesbetween antigens and immunogens? (

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1,. )ntigens are targets of an immune lesponse, while immunogens induce an une response' -8. Immunogens induce cellular immunity, while antigens induce humoral immunity. C. In order to induce an immune response,immunogensneedto be coupledto a foreign protein "carrier", while antigenscan be injected alone' D. All of the above are true statcments E. Only A and B are correct

2. The membraneprotein on an antigenpresentingcell which is usedto bind and present antigen to helper T cells is --.

T cell receptor Class II

MHC Class I IgA CD4 3. Which of the following are true statementsconcerning clonal selection? A. B. , C* (y) E.

Each lymphocyte generatesone unique antigen-bindingspecificity Self-reactive lymphocytes are deleted Foreign-reactive lymphocytes proliferate and differentiate All of dre above are true statements Only A and B are correct

4. Which of the following are true statementsconcerningimmunoglobulin isotypes?

(' 1

' \A--The isotype of an immunoglobulin is determined by the type of heavy chain that it contains. B. Isotype switching refers to a changein the antigenspecificity of eachisotype. C. The isotype of an immunoglobulin is determinedby the light chain gene locus (either r or L). D. AU of the above are true statements E. Only A and B are correct 5. The effector molecule of the humoral immune system which is transported across epithelia and into secretoryproducts such as saliva is A. IgM _--8. CD8

f,e) lge Y

"'

-*--:

invariant chain E. IgE

6. Which of the foliowins best describesthe differencesbetweenrecombinationof immunoglobulin heavy u"'a figfrt chain genes? A. Heavy chain genesrearrangeDNA sequences,while light chain rearrangementis _ performed by RNA processing. Heavy chain genesencodeV, D, and J segmentsthat rearrange,while light chain €) v rearrangementusesonly V and J gene segments. C. Heavy chain genesundergo rearrangementafter light chain genes. D. Heavy chain, but not light chain generearrangementusesmechanismsof junctional diversity. E. The heavy chain genesare encoded on two different chomosomes,while light chain genesare encodedon one.

i' immuno quiz 1+ answers,2002

StronaI z

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Quiz l with answers thanthe innateimmuneresponse in the is moreessential 1. True/False The acquiredimmuneresponse defenseagainstmicrobes. 2.Whichof the followingbestdescribesthe differences betweenhowT cellsand B cellsrecognize antigen? solubleantigen. whileB cellsrecognize A. T cellsrecognize selfMHCmolecules, B. T cells recognizeforeign peptidesin the contextof self MHCmolecules,while B cells recognizesolubleantigen. antigens. non-protein proteinantigens, whileB cellsrecognize C. T cellsrecognize proteins. whole whileB cellsrecognize foreignpeptides, D. T cellsrecognize peptideantigens. whileB cellsrecognize selfMHCmolecules, E. T cellsrecognize of diversityat the the generation concerning 3. Whichof the followingare truestatements immunoglobulin chainlocus l:uuY occursbeforea B cellis exposedto antigen A. Somatichypermutation B. lsotypeswitchingoccursbeforea B cellis exposedto antigen C. P- and N-nucleotides are addedto D-J genejunctionsbeforea B cell is exposedto antigen All the of abovearetrue statements D. E. OnlyA and B are correct is madeup of 6 hypervariable molecule 4. True/FalseEachantigenbindingsiteof an immunoglobulin loops. S.Theprocessby whichneutrophilscrossthe vascularendotheliumis called A. B. C. D. E.

phagocytosis chemotaxis complementfixation diapedesis acutephaseresponse

6. Interferons are inducedin a viralinfectionby the presenceof A. B. C. D. E.

singlestrandedRNA singlestrandedDNA double stranded RNA All of the above Noneof the above

ansrvers.htm I A2-quizIYo2B http://medocs.ucdavis. edu/muril.t8 0a/exams

2005-05-